CRISPR

CRISPR Knockout in mammalian cells,

CRISPR-Cas9 is an emerging new tool for editing genes. In this method, 20bp single guide RNA (sgRNA), designed to target a specific site in the genome, associates with Cas9 protein to create a double strand break (DSB) in the DNA. Once a DSB is created it can be repaired by either non-homologous end joining (NHEJ) or homology directed repair (HDR) pathways.  In the NHEJ pathway the DSB is randomly rejoined, with increased probability for random indels to occur, thus creating a frameshift which may lead to gene knockout. The HDR pathway, on the other hand, uses a homologues DNA template to precisely correct the DSB, thus allowing to perform knockin mutation.

We offer two service modes for CRISPR-Cas9 application to knockout genes in cell lines:

  1. Full- from design step to knockout cells. At the end we will provide two freeze vials of knockout clone.
  2. Consultation- includes sgRNA and primers’ design together with guidance along the process (PCR for screening, Sanger) and reagents for start. It for researchers who wish to perform CRISPR in their own lab.

 

For orders please fill and send us the CRISPR application form

For additional information, please contact Liat Linde, tel 04-8295452/221.