CRISPR Knockout in mammalian cells,
CRISPR-Cas9 is an emerging new tool for editing genes. In this method, 20bp single guide RNA (sgRNA), designed to target a specific site in the genome, associates with Cas9 protein to create a double strand break (DSB) in the DNA. Once a DSB is created it can be repaired by either non-homologous end joining (NHEJ) or homology directed repair (HDR) pathways. In the NHEJ pathway the DSB is randomly rejoined, with increased probability for random indels to occur, thus creating a frameshift which may lead to gene knockout. The HDR pathway, on the other hand, uses a homologues DNA template to precisely correct the DSB, thus allowing to perform knockin mutation.
We offer two service modes for CRISPR-Cas9 application to knockout genes in cell lines:
For orders please fill and send us the CRISPR application form