The Zeiss LSM 880 with Airyscan is a new generation confocal instrument for more sensitivity, faster linear scanning and super-resolution (x1.7 improvement) imaging.
The system features a cooled GaAsP detector and two detectors that complement the spectral working range enabling flexible imaging strategies, including molecular dynamics, recording fluorescence at single photon signal levels and sequential spectral acquisition followed by unmixing of overlapped emission signals.
With the Airyscan detector the system achieves resolution enhancement by use of a radial array of 32 GaAsp detectors which utilizes light that otherwise would be rejected by the confocal pinhole, increasing signal to noise ratio by x4 to x8 times which is utilized to further speed up scanning, get higher sensitivity or increase resolution (by a factor of x1.7) in all 3 dimensions.
In Fast Airyscan mode, four pixel columns are simultaneously imaged utilizing 16 detector elements of the Airyscan detector’s center resulting in x4 faster acquisition with x4 improvement in SNR and x1.5 improvement in resolution.
|Argon multiline||458, 488, 514nm|
|Objective lens||Magnification||NA||coverslip||Working distance||Immersion||Parfocal length|
|Plan Apochromat||x10||0.45||0.17 mm||2 mm||Air||45 mm|
|Plan Apochromat||x20||0.8||0.17 mm||0.55 mm||Air||45 mm|
|W Plan Apochromat||x20||1.0||–||1.8 mm||Water dipping||75 mm|
|W Plan Apochromat||x40||1.0||–||2.5 mm||Water dipping||45 mm|
|Plan Apochromat||x63||1.4||0.17||0.19 mm||Oil||45 mm|
Acquisition is performed with the Zeiss proprietary ZEN 2.3 system software.
The Zen software provides a wide range of 2D/3D image processing tools: export to TIFF, maximum projection, 3D reconstruction, 2D colocalization, spectral unmixing and more. For basic visualization and processing you can download the free ZEN lite edition software. An offline full version is available for analysis at Computer Image-Pro-Prem. Output files can be opened by Imaris and ImageJ/FIJI for analysis.
Please contact Edith Suss-Toby, tel. +972-4-8295347 to coordinate a meeting.
Tips for immunofluorescence experiments.
Please prepare controls before starting a new model.