Introduction
The PerkinElmer IVIS 200 imaging system provides high throughput non-invasive quantitative fluorescence and bioluminescence (luciferase activity) imaging.
The IVIS 200 has the capacity to image up to 5 mice or 2 rats simultaneously, ex-vivo specimens and cell culture plates. The system is equipped with an isoflurane anesthesia system, low-autofluorescence optics and a cooled CCD camera (‑90°C) for a maximum resolution of 60µm.
Available fluorescence filters
| Reflector Turret | Name | Excitation (nm) | Emission (nm) | Background (nm) |
|---|---|---|---|---|
| 1 | GFP | 445-490 | 515-575 | 410-440 |
| 2 | DsRed | 500-550 | 575-650 | 460-490 |
| 3 | Cy5.5 | 615-665 | 695-770 | 580-610 |
| 4 | ICG | 710-760 | 810-875 | 665-695 |
Spectral imaging filters
| Reflector Turret | Name | Excitation (nm) | Emission (nm) | Background (nm) |
|---|---|---|---|---|
| 5 | 560nm | 550-570 | ||
| 6 | 580nm | 570-590 | ||
| 7 | 600nm | 590-610 | ||
| 8 | 620nm | 610-630 | ||
| 8 | 640nm | 630-650 | ||
| 8 | 660nm | 650-670 |
* To decrease autofluorescence it is recommended to use far-red emitting antibodies or dyes.
Applications
- Quantitative longitudinal measurement of tumor development, metastasis, angiogenesis, and response to therapy
- Trafficking of cellular and other biological therapies (e.g., immune cell, stem cell, or viral therapeutic platforms or antibodies, peptides, metabolites, etc.)
- Monitoring of host immune cell responses
- Distribution, quantification, and kinetic analyses of gene expression or enzymatic activity in vivo
- For more information see the video:
Analysis
The IVIS 200 dedicated Living Image 3.2 software allows absolute quantification (photons/sec/cmsq/sr) of bioluminescence and fluorescence signal, for in vivo, ex vivo and in vitro studies. It supports automatic ROI creation, background subtraction, scaling and quantification. The software is available both at the IVIS station and at the BCF image analysis unit on the Computer in-vivo analysis .
New users
Please contact Edith Suss-Toby, tel. +972-4-8295347/61 to coordinate a meeting.
Reading materials prior to the first instruction session:
Quantification of signal from IVIS
IVIS 200 operation instructions (Hebrew)
The system can be reserved in the BookItLab online ordering system, In-vivo imaging IVIS 200 System; luciferin aliquots (15mg/ml) can be ordered in BookItLab, under Other Services; select Offline service type: Luciferin tube
Protocol for in vitro bioluminescence assay
Protocol for in vivo bioluminescence assay
