The Cytometry Center at the Technion’s Faculty of Medicine is equipped with a BD LSRFortessa system. This cell analyzer delivers high-performance multicolor analysis and is configured with four lasers; UV 355, Violet 405, Blue 488 and Red 640. It can detect up to 17 parameters to support ever increasing demands in multicolor flow cytometry.
For filters and channel information see here.
The instrument is available for trained users in a self-operated mode. Untrained users must contact core personnel to schedule hands-on training.
Pre-reservation is required and can be done via BookIt. Please note, the LSRFortessa can get busy at times, it is therefore best to reserve it as early ahead of time as possible.
Acquisition is done with BD FACSDiva™ v7 software and FCS files should be exported to our bcf-server after acquisition. Please make sure to backup you own data – we are not doing so!
Data analysis of the FCS files can be done with any third party analysis software. We have several working stations with a variety of software. See our Data Analysis page for additional information.
Please see our Fortessa instructions file.
For further information or if you need assistance please contact core personnel.