The Rotor-Gene 6000 (Qiagen) is designed to perform real-time and end-point PCR and high-resolution melting (HRM) analysis. It is based on centrifugation, which prevents condensation and removes air bubbles. Furthermore, there is fixed path of fluorescent optical on the side of the chamber, which ensures consistent excitation for every sample. It eliminates the need for a passive internal reference dye such as ROX.
Following our guidelines, you will be able to independently work with this system, or if you prefer, we can provide you full service on this system or alternatively on ABI7300 Real-Time PCR system. Full service for gene expression and genotyping applications includes primer design, reactions with either, SybrGreen or TaqMan probes, and data analysis.