3DHistech Pannoramic 250 Flash III is an automatic slide scanner for histology and fluorescence with 250 slide capacity for analysis, telepathology and e-learning. Brightfield scanning is performed very fast by flashing a stroboscope light source and continuous stage movement.
This is a proprietary system.
Objective | Magnification/NA | Pixel size Brightfield | Pixel size Fluorescence |
---|---|---|---|
Plan-Apochromat | x40/0.95 | 0.121μm | 0.101 μm |
Plan-Apochromat | x20/0.80 | 0.242μm | 0.203 μm |
Camera | Technology | Application | Resolution | Data depth |
---|---|---|---|---|
Adimec Q12A180 | CMOS | Brightfield | 4096 x 3072px | 12 bit |
pco.edge 4.2 | sCMOS | Fluorescence | 2048 x 2048px | 16 bit |
Brightfield: Xenon flashlamp
Fluorescence: Lumencor SPECTRA Light Engine 6
LED | Excitation filter | Emission filter | Fluorophore family |
---|---|---|---|
386/23 | 387/18 | 440/44 | DAPI |
438/24 | 436/20 | 480/40 | CFP |
475/28 | 485/26 | 521/27 | Cy2 |
550/88 | 559/32 | 607/40 | Cy3, Texas Red |
650/13 | 649/19 | 700/57 | Cy5 |
720/30 | 708/75 | 809/81 | Cy7 |
All fixed specimen brightfield/widefield applications including FISH and rare event.
Please read instructions on preparation of slides here.
Acquisition is performed using the proprietary Pannoramic scanner software. Visualization and analysis are performed using the CaseViewer software, free to download from the 3DHISTECH site, providing seamless zooming and panning of the virtual slide and export to JPEG and TIFF for quantitative analysis in Imaris or ImageJ/FIJI.
Please contact Maya Holdengreber, tel. +972-73-378-1107 to coordinate a meeting.