Introduction
The Life Canvas Technologies SmartSPIM is an advanced lightsheet microscope for imaging large cleared tissue samples with high speed and resolution. Its patented axial sweeping technology illumination optics scan the beam while synchronized to the camera’s rolling shutter detection, providing homogeneous axial PSF across the entire field of view.
Hardware
The SmartSPIM is designed from the ground up. Motorized XYZ stage and stacked Z stage, 15mm wide by 25mm long sample holders, sCMOS camera Hamamatsu Fusion C14440.
Laser lines and filter sets
405nm, 488nm, 561nm, 642nm.
| Laser line | Filter set | Fluorophores group |
| 642 | 680/42 | Alexa 647 ,Cy5 |
| 561 | 600/52 | Alexa 555, Cy3 mCherry |
| 488 | 525/50 | GFP, Alexa 488, Cy2 |
| 405 | 445/58 | DAPI, Alexa 405, BFP |
Objective lenses
| Objective lens | Magnification | NA | Working distance | EFL | |
| Thorlabs TL4X-SAP |
x3.6 | 0.2 | 17mm | 60mm | |
| Special Optics 54-10-12 |
x16.7 | 0.4@RI1.45 | 12mm | 12mm | RI 1.33-1.56 |
| Special Optics 54-12-8 |
x23.8 | 0.7@RI1.45 | 10mm | 8.4mm | RI 1.33-1.56 |
Acquisition
The acquisition is performed with the SmartSPIM acquisition system software.
Analysis
The system has a unique destriping and stitching GUI.
New users
Please contact Maya Holdengreber, tel. +972-73-3781106, to coordinate a meeting.
Reservations
For lightsheet imaging, the SmartSPIM can be booked following instruction at our reservation system
