The system features two cooled GaAsP detectors for emissions ranging from 400 to 700nm and a transmitted light detector. With the Airyscan 2 detector, the system achieves resolution enhancement by use of a radial array of 32 concentrically arranged detection elements which utilizes light that otherwise would be rejected by the confocal pinhole which remains open, increasing the signal to noise ratio by x4 to x8 times (120nm lateral, 350nm axial). The split signal is deployed to further speed up scanning, get higher sensitivity, or increased resolution. The Multiplex mode for Airyscan 2 adds new detection configurations for parallel pixel acquisition for fast and gentle acquisition on live samples.
The LSM900 is attached to an upright microscope (Axio Examiner Z1). The stage carrier is fixed.
|640 nm||Cy5, APC, Alexa 647, TO-PRO-3|
|561 nm||Cy3, TAMRA, PI, Texas Red, PKH26|
|488 nm||Cy2, GFP, Mitotracker GreenOhter|
|405 nm||DAPI, Hoechst|
|Position||Objective lens||Magni-fication||NA||coverslip||Working distance||Immersion||Parfocal length|
|1||W Plan Apochromat||x20||1.0||0||1.8 mm||Water dipping||75 mm|
|2||W Plan Apochromat||x10||0.5||0||3.7 mm||Water dipping||75mm|
|3||EC Plan Neofluar||x5||0.16||0.17mm||18.5mm||Without||45mm|
|4||W Plan Apochromat||x40||1.0||0||2.5 mm||Water dipping||45 mm|
Objectives from one of our other systems can be installed for a particular experiment by appointment.
Acquisition is performed with the Zeiss proprietary ZEN 3.0 system software.
The Zen Blue 3.0 software provides a wide range of 2D/3D image processing tools: export to TIFF, maximum projection, 3D reconstruction and more. For basic visualization and processing, you can download the free ZEN lite edition software.
An offline full version is available for processing and analysis at Computer CT software. Output files can be opened by Imaris and ImageJ/FIJI for analysis.
Please contact Maya Holdengreber, tel. +972-77-8871106, to coordinate a meeting.
Tips for immunofluorescence experiments.
Please prepare controls before starting a new model.