Odyssey Infrared Imaging System
The Odyssey infrared imaging scanner equipped with two lasers:
Solid-state diode laser at 685 nm and Solid-state diode laser at 785 nm.
The emission light at 700nm and 800nm is suitable for wide-range of applications .
- Protein Staining- IRDye® Blue Protein Stain is a strong 700 nm fluorophore, and its fluorescence is induced upon protein binding.
- NUCLEIC ACID GEL DOCUMENTATION-Sensitivity is high and background fluorescence is low when using SYTO® 60 red fluorescent nucleic acid stain to visualize DNA gels on the LI-COR Odyssey® Infrared Imagers.
- EMSA/GEL SHIFT ASSAY-IRDye end-labeled oligonucleotides and the Odyssey® CLx Infrared Imaging System provide a safe and sensitive alternative to radioactive EMSA detection.
- IN-CELL WESTERN™ ASSAY- A quantitative immunofluorescent assay for simultaneous analysis of two different proteins in fixed cultured cells.
- QUANTITATIVE WESTERN BLOTTING-For detection, replace your HRP-conjugated antibody with secondary antibodies labeled with IRDye infrared dyes. Two protein targets can be detected in separate fluorescent channels, even if the bands co-migrate. Better image clarity for sharper, more detailed bands. Detect subtle mobility shifts caused by protein modifications such as phosphorylation.
- RNAi ANALYSIS- Quantitative Western blotting is an essential tool for every step in your RNAi studies: Choosing an RNAi vehicle (siRNA or shRNA or other RNA-producing vector construct), Confirming level of knockdown of the target gene, Determining the impact of target gene knockdown on a phenotype such as cell proliferation, cell migration, cell cycle, or cell signaling pathways.
- TISSUE SECTIONS-The Odyssey Systems can image tissue sections stained with IRDye antibodies or optical probes. The sections can be labeled with standard immunohistochemical protocols or in vivo as part of an animal imaging study. Sections are imaged at 21 µM resolution for macro-level analysis. Many sections can be imaged in a single scan for screening, so that valuable microscope time is used more efficiently.