Time Lapse 1 is an inverted system for transmitted and fluorescence widefield microscopy of fixed specimens and short or extended time lapse of live samples.
The Zeiss Axio observer microscope in Time lapse 1 system is wholly enclosed in a polycarbonate incubator system with controlled temperature and humidity and an active gas mixer. It features a motorized stage, X-cite metal halide light source and monochrome AxioCam HRm and high speed Axiocam HS.
Reflector Turret | Excitation | Beamsplitter | Emission | Suitable fluorophores |
---|---|---|---|---|
Analyzer module DIC | ||||
Zeiss Filter set 50 (optional) | BP 640/30 | FT 660 | BP 690/50 | Cy5, DRAQ5 |
Zeiss Filter 20HE | BP 546/12 | FT 560 | BP 607/80 | PI, Cy3, Alexa555, Rhodamine |
Zeiss Filter set 38HE | BP 470/40 | FT 495 | BP 525/50 | Cy2, GFP, Alexa 488 |
Zeiss Filter set 49 | G 365 | FT 395 | BP 445/50 | DAPI, Hoechst |
Optovar x1.6 |
Objective | Magnification | NA | Contrast | Dish bottom or slide coverslip | Working distance (mm) | Immersion |
---|---|---|---|---|---|---|
EC Plan-Neofluar | x5 | 0.16 | Phase 1 | plastic/glass | 18.5 | air |
EC Plan-Neofluar | x10 | 0.3 | Phase 1 | plastic/glass | 5.2 | air |
Plan-Neofluar | x20 | 0.4 | Phase 2 | plastic/glass | 7.4 | air |
Apochromat | x20 | 0.8 | Phase 2 & DICII | Glass | 0.55 | air |
Apochromat | x40 | 0.95 | Phase 3 | Glass | 0.25 | air |
Plan-Apochromat (optional) | x63 | 1.4 | Phase 3 | Glass | 0.19 | oil |
We have glass bottom dishes for you to try imaging with the higher end objectives
Acquisition is performed with the Zeiss proprietary Zen software. Output files can be opened by the ZEN lite edition software, ImageJ/FIJI or exported to TIFF for analysis.
The Zen 2 software provides fast acquisition, tiling and positions, multidimensional and fast acquisition and basic 2D analysis.
Long term live experiments in 6 dimensions: XYZ, positions, channels, time, in optimal gas composition or hypoxia. Also FRET, FRAP, wound healing, tube formation.
For basic visualization and processing you can download the free ZEN lite edition software. Output files can be opened by ImageJ/FIJI and Imaris for analysis.
Please contact Maya Holdengreber, tel. +972-73-3781107, to coordinate a meeting.
Tips for immunofluorescence experiments.
Please prepare controls before starting a new model.
For reference you may use the Time Lapse 1 instruction file .
For long term live widefield microscopy, Time Lapse 1 can be reserved in the BookItLab online reservation system, Microscope Time-lapse 1.