The Bruker Ultima 2-Photon Plus is an upright multiphoton microscope system designed for deep tissue imaging and intravital microscopy. This system combines tunable and fixed laser sources with high-sensitivity detection for applications including second harmonic generation (SHG) and deep tissue penetration up to 700 μm.

System Specifications

Laser Sources:

Spectra-Physics InSight X3 DA: Tunable laser (680–1300 nm)

Fixed output: 1045 nm

Detection System:

Detectors: Four GaAsP PMTs

Emission channels:

660/40 nm (e.g., Cy5, Alexa Fluor 640)

595/50 nm (e.g., mCherry, mScarlet, Cy3)

525/50 nm (e.g., GFP, Cy2)

460/50 nm (e.g., DAPI, Atto 405)

Scanning System:

Galvo scanners: 6mm imaging galvanometers with motorized Z focus

Resonant scanner: High-speed (30 fps at 512 × 512 FOV)

Field of view: 1.11mm x 1.11mm standard, 1.4mm x 1.4mm overscan

Objectives:

10x/0.3 Water immersion 3.5 mm WD

20x/1.0 Water immersion 2 mm WD

40x/0.75 Water immersion 2.5 mm WD

Advanced Features:

ETL remote focusing: Decoupled 10mm range for independent Z positioning

Multi-channel acquisition: Simultaneous acquisition of 2 channels using both lasers

Stage inserts: Compatible with standard slides and Petri dishes

Applications

Intravital Microscopy:

Vascular dynamics studies

Calcium imaging in living tissue

Neuronal activity mapping

Second Harmonic Generation (SHG):

Collagen fiber imaging

Tissue structure analysis

Label-free imaging

Deep Tissue Imaging (up to 700 μm penetration)

Spheroid\Organoids

Organs

3D Printed tissue

New users

Please contact Maya Holdengreber, tel. 073-378-1107 to coordinate a meeting.