Example: Giardia under oxidative stress
Untargeted metabolomicsUntargeted lipidomicsMulti-omicsUnpublished data
How does the anaerobic parasite Giardia lamblia respond when it meets oxygen?
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What we did
Trophozoites were grown anaerobically or exposed to O2 (n = 4 each). The same eight cell pellets were measured by untargeted metabolomics (662 metabolites) and lipidomics (575 lipid species) at our center, and by transcriptomics and proteomics at the Technion Multi-Omics Center, which integrated all four layers.
What we found
Oxygen shuts down Giardia’s anaerobic core. Small-molecule pools fall (pyruvate, glyceraldehyde 3-phosphate, 2-oxoglutarate and the phospholipid precursors DHAP, glycerol 3-phosphate, serine and choline), while membrane lipids go the other way: 136 lipids rise, led by PE (all 19 changed PE species are up), and lyso-PC falls. Membranes are remodelled rather than built anew, and transcripts and proteins shift along the same axis.
In collaboration with Prof. Elah Pick, Tel-Hai College. Multi-omics integration: Dr. Dor Shalev, Dr. Oz Solomon and Dr. Eilon Barnea, Technion Multi-Omics Center.
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