Other Slide Scanners
Slide Scanner 250 Flash III
![](https://bcf.technion.ac.il/wp-content/uploads/2024/03/ima-SlideScanner-Flash-III.jpg)
Introduction
3DHistech Pannoramic 250 Flash III is an automatic slide scanner for histology and fluorescence with 250 slide capacity for analysis, telepathology and e-learning. Brightfield scanning is performed very fast by flashing a stroboscope light source and continuous stage movement.
Hardware
This is a proprietary system.
Light sources
Brightfield: Xenon flashlamp
Fluorescence: Lumencor SPECTRA Light Engine 6
LED | Excitation filter | Emission filter | Fluorophore family |
---|---|---|---|
386/23 | 387/18 | 440/44 | DAPI |
438/24 | 436/20 | 480/40 | CFP |
475/28 | 485/26 | 521/27 | Cy2 |
550/88 | 559/32 | 607/40 | Cy3, Texas Red |
650/13 | 649/19 | 700/57 | Cy5 |
720/30 | 708/75 | 809/81 | Cy7 |
Objectives
Objective | Magnification/NA | Pixel size Brightfield | Pixel size Fluorescence |
---|---|---|---|
Plan-Apochromat | x40/0.95 | 0.121μm | 0.101 μm |
Plan-Apochromat | x20/0.80 | 0.242μm | 0.203 μm |
Cameras
Camera | Technology | Application | Resolution | Data depth |
---|---|---|---|---|
Adimec Q12A180 | CMOS | Brightfield | 4096 x 3072px | 12 bit |
pco.edge 4.2 | sCMOS | Fluorescence | 2048 x 2048px | 16 bit |
Applications
All fixed specimen brightfield/widefield applications including FISH and rare events.
Slide preparation
Please read the instructions on the preparation of slides here.
Acquisition and analysis
Acquisition is performed using the proprietary Pannoramic scanner software. Visualization and analysis are performed using the CaseViewer software, free to download from the 3DHISTECH site, providing seamless zooming and panning of the virtual slide and export to JPEG and TIFF for quantitative analysis in Imaris or ImageJ/FIJI.
New users
Please contact Maya Holdengreber, tel. 073-378-1107 to coordinate a meeting.
Please do not make a reservation through our online BookItLab system.