Slide Scanner Slideview VS200

Introduction

The Olympus Slideview VS200 is a slide scanner for high-throughput digitalization of histology and fluorescence slides for archival, diagnostics, teaching, and research. It supports five imaging modes: Brightfield, Darkfield, Phase Contrast, Polarization, and Fluorescence.

The system’s automatic slide loader can hold standard slides (26 × 76 mm). Trays for even larger slides (up to 102 x 127 mm) are also available and can be purchased if there is a need. High-resolution images are converted into virtual slides through an automated high-speed scanning and tiling process.

Hardware

This is a proprietary system.

Light source and filters

Excelitas X-Cite NOVEM 9-Channel LED Illumination System.

Fluorescence Filters

AHF Analyzenteknich pentaband filter

LED	Excitation filter	Emission filter	Fluorophore family
386/23	352-404	416-452	DAPI
475/28	460-488	500-530	FITC
550/88	542-566	593-607*	Cy3, mCherry
650/13	626-644	665-705	Cy5
720/30	721-749	767-849	Cy7

Objectives

Objective	Magnification/NA	Working Distance	Pixel size Brightfield	Pixel size Fluorescence
Universal Plan Extended Super Apochromat	x10/0.4	3.1 mm	548 nm	548 nm
Universal Plan Extended Apochromat	x20/0.80	0.6 mm	273.8 nm	325 nm
Universal Plan Extended Super Apochromat	x40/0.45	0.18 mm	137 nm	172.5 nm

Cameras

Camera	Technology	Application	Resolution	Data depth
iDS VS-264C	CMOS	Brightfield
CAM-ORCA-FusionBT Digital Hamamatsu	sCMOS	Fluorescence	2304 x 2304 px	16 bit

Applications

All fixed specimen brightfield/widefield applications including FISH and rare events, polarized light, phase contrast, darkfield. One plane, z-stack or extended focus acquisition.

Acquisition and analysis

Acquisition is performed using the proprietary CellSens software. The .vsi virtual slide image files can be opened by Olyvia, QuPath, FIJI software.

New users

Please contact Maya Holdengreber, tel. 073-378-1107 to coordinate a meeting.
Please do not make a reservation through our online BookItLab system.