Cell Phenotype

• Immunological Genome Project (ImmGen)
• OMIPs (Optimized Multicolor Immunofluorescence Panel) – a special peer-reviewed Cytometry Part A publication type that reports on newly designed and optimized multicolor panels for flow cytometry, fluorescence microscopy, image cytometry, and other polychromatic fluorescence-based methods.

Spectral Viewers

• BD Fluorescence Spectrum Viewer
• Invitrogen Spectra Viewer
• BioLegend Spectra Analyzer

Multicolor Flow Cytometry

• FluoroFinder
• Life Technologies School of Fluorescence
• Introduction to Fluorescent Proteins (Nikon Microscopy)
• Quick-guide-to-designing-multicolor-panels (BioLegend)
• Autofluorescence: Causes and Cures

Tutorials

• Basic Introduction to Flow Cytometry
• Fluorescence Tutorials (Life Technologies)
• FlowJo Training (Tutorials)
• Paul Robinson’s Purdue Class
• Other lectures on the Purdue Cyto site
• Interpreting Flow Cytometry Data: A Guide for the Perplexed (Herzenberg et al. 2006 Nature Immunology 7:681)

Software

• FlowJo
• DeNovo Software (FCS Express)
• Verity Software House (ModFit, WinList, GemStone)
• Cytobank

Freeware

• SPICE and PESTLE for analysis of high content multivariate data sets (Mac OS)
• Cytospec (Purdue Software)

Vendor Websites

• BD Biosciences
• Beckman Coulter
• Cytek Development
• Intellicyt
• Invitrogen
• eBiosciences
• BioLegend
• Spherotech
• Phoenix Flow
• Novus Biologicals
• R&D Systems

Cytometry Groups

• International Society for Analytical Cytology (ISAC)
• Purdue Flow Cytometry (current flow issues and concerns by users over the globe)