High Throughput LSRFortessa

High Throughput LSRFortessa

The Cytometry Center at the Technion’s Faculty of Medicine has acquired a BD LSRFortessa system. This cell analyzer delivers high-performance multicolor analysis and is configured with five laser lines: UV 355, Violet 405, Blue 488, Yellow Green 561, and Red 640. It can detect up to 18 fluorescent parameters. The instrument is equipped with a High Throughput Sampler (HTS), allowing the acquisition of data from 96 or 384 well plates.

For information on filters and channels, see here.

The instrument is available for trained users in self-operated mode. Untrained users must contact core personnel to schedule hands-on training.

Pre-reservation is required and can be done via BookItLab.

Acquisition is done using BD FACSDiva v7 software. FCS files should be exported to the bcf-server after acquisition. Please make sure to back up your own data, as we do not provide backup services!

Data analysis of the FCS files can be done with any third-party analysis software. We have several workstations with a variety of software available. See our Data Analysis page for additional information.

For further information or assistance, please contact Amir Grau.