Flow Cytometry RNA Assay reveals the dynamics of RNA and protein expression within individual cells, facilitating unprecedented analysis of their correlation as the cell changes over time or in response to stimulus. This novel assay employs a proprietary fluorescent in situ hybridization (FISH) technique enabling simultaneous detection of up to three RNA transcripts in a single cell using a standard flow cytometer. RNA detection may be combined together with intracellular and cell surface antibody staining to elevate the understanding of single cell dynamics to a new dimension.
Coupling RNA expression with protein detection on a flow cytometer generates multiparametric data in heterogeneous cell populations and offers in-depth and high content details at the single cell level. In contrast, microarrays and sequencing can provide comprehensive gene expression data in bulk sample preparations; however, such bulk sample preps can mask the individual effects of different cellular subsets. Using the PrimeFlow™ RNA Assay, specific cell populations may be analyzed for unique transcript expression levels or cell subsets evaluated over time to determine transcriptional regulation and protein expression simultaneously. Such unique and valuable insights are highly applicable to answering previously unanswerable questions and have broad implications for advancing research across multiple fields of biology.
Development of the PrimeFlow™ RNA Assay is based upon proven and well-published ViewRNA™ technology designed for microscopic analysis of RNA in cells and tissues. The assay combines paired oligonucleotide probe design with branched DNA (bDNA) signal amplification to robustly detect up to three RNA transcripts at the single-cell level using a standard flow cytometer.