Confocal LSM 700

Confocal LSM 700


The Zeiss LSM700 is a laser-scanning confocal system attached to an inverted motorized microscope (Axio Observer). Its variable secondary dichroic beamsplitter splits emission signals between the two PMTs and the cutoff can be freely selected in 1nm steps. For short-term live experiments, the system can be optionally equipped with a heating stage insert for 35mm plates.



Line Wavelength Power Suitable fluorophores
Solid state laser cassette 633 nm 5mW Cy5, APC, Alexa 647, TO-PRO-3
Solid state laser cassette 555 nm 10mW Cy3, TAMRA, PI, Texas Red, PKH26
Solid state laser cassette 488 nm 10mW Cy2, GFP, Mitotracker Green, JC1
Solid state laser cassette 405 nm 5mW DAPI, Hoechst



Lens Magnification NA coverslip Working distance Immersion
EC Plan Neofluar x10 0.3 0.17 mm 5.2 mm Air
Plan Apochromat x20 0.8 0.17 mm 0.55 mm Air
LD LCI Plan Apochromat x25 0.8 0-0.17 mm 0.57 mm Oil Glycerin Water
LCI Plan Neofluar (optional) x25 0.8 0-0.17 mm 0.21 mm Oil Glycerin Silicone Oil Water
EC Plan Neofluar x40 1.3 0.17 mm 0.21 mm Oil
Plan Apochromat x63 1.4 0.17 mm 0.19 mm Oil



Acquisition is performed with the Zeiss proprietary ZEN software. Two PMT detectors for simultaneous detection, and up to four sequential tracks and one transmitted-light PMT allow superimposing fluorescence images on brightfield or DIC images.
The software allows 360° XY scanning field rotation, and freely definable regions of interest (ROI).
Acquisition modes: Spot, line/spline, frame, lambda stack.
Resolution up to 2048 x 2048 pixels, scanning speed up to 5 frames/sec at 512 x 512 pixels.


Special Applications

Photobleaching, short-term live cell imaging, ROI, positions, tiling, time series and combination (XYZ|T), increased intensity over Z.



The Zen software provides a wide range of 2D/3D image processing tools: export to TIFF, maximum projection, 3D reconstruction, 2D colocalization, spectral unmixing, tile stitching and more. For basic visualization and processing, you can download the free ZEN lite edition software. Offline full versions are available for analysis at CT Computer and Computer IMARIS software. Output files can be opened by Imaris and ImageJ/FIJI for analysis.

New Users

Please contact Maya Holdengreber, tel. 073-3781107, to coordinate a meeting.
Tips for immunofluorescence experiments.
Please prepare controls before starting a new model.



The Confocal LSM 700 inverted microscope can be reserved through the BookItLab online reservation system.