Confocal LSM 900 Inverted

Confocal LSM 900 Inverted


The Zeiss LSM 900 with Airyscan 2 is a confocal instrument with super-resolution (x1.7 improvement) imaging. The system features two cooled GaAsP detectors for emissions ranging from 400 to 700nm, and a transmitted light detector. With the additional Airyscan 2 detector, the system achieves resolution enhancement (120nm lateral, 350nm axial) from light that would otherwise be rejected by the confocal pinhole that remains open, increasing the signal-to-noise ratio by 4 to 8 times. The split signal is deployed to further speed up scanning, get higher sensitivity, or increase resolution in all 3 dimensions. The Multiplex mode for Airyscan 2 adds new detection configurations for tandem pixel acquisition for fast and gentle acquisition on live samples.

LSM Plus is a complementary imaging function in confocal mode that optimizes acquisition settings and performs linear deconvolution to yield higher resolution and improved SNR.

The system stands on an anti-vibration table and has a top-stage incubation system with temperature and CO2 control and a high-performance control computer.



The LSM900 is attached to an inverted motorized stage microscope (Axio Observer 7) with Definite Focus 3 which is a focus finder that eliminates drifting.



Wavelength Suitable fluorophores
Solid state laser cassette 640 nm Cy5, APC, Alexa 647, TO-PRO-3
Solid state laser cassette 561 nm Cy3, TAMRA, PI, Texas Red, PKH26
Solid state laser cassette 488 nm Cy2, GFP, Mitotracker Green, JC1
Solid state laser cassette 405 nm DAPI, Hoechst


Objective lenses 

Position Objective lens Magni-fication NA coverslip Working distance Immersion Parfocal length
1 EC Plan Neofluar x5 0.15 0.17mm 18.5mm Without 45mm
2 Plan-Apochromat x10 0.45 0.17mm 20mm Without 45mm
3 Plan-Apochromat x20 0.8 0.17mm 0.55mm Without 45mm
4 LD LCI Plan-Apochromat x25 0.8 0.17mm 0.57 at cover glass 0.17 Water, Silicone Oil, Glycerine, Oil 45mm
5 Plan-Apochromat x63 1.4 0.17mm 0.19mm oil 45mm



Acquisition is performed with the Zeiss proprietary ZEN Blue 3.5 system software.



The Zen Blue software provides a wide range of 2D/3D image processing tools: export to TIFF, maximum projection, 3D reconstruction, 2D colocalization, image stitching and more. For basic visualization and processing, you can download the free ZEN lite edition software. Offline full versions are available for analysis at CT Computer and Computer IMARIS software. Output files can be opened by Imaris and ImageJ/FIJI for analysis.


New users

Please contact Maya Holdengreber, tel. 073-378-1106, to coordinate a meeting.
Tips for immunofluorescence experiments.
Please prepare controls before starting a new model.



For general and live cell confocal imaging including tiling and z sections, the LSM900 Inverted can be reserved through our BookItLab online reservation system.

Reading Material Before the First Instruction Session