Pratibha Ahirwal
Matteo Ghiringhelli
Matteo Ghiringhelli
Stereomicroscope
Introduction
The Olympus MVX10M research macro zoom microscope images brightfield and fluorescent specimens, such as Drosophila larvae, organs of small animals, and more.
Hardware
Camera
DP73 is a 17.28 megapixel color CCD (2 megapixels with pixel shifting), 12bit
1600×1200 pixel images at 15 fps, 800×600 at 27fps
Objectives
| Objective | Magnification/NA | Working distance (mm) | Total magnification |
|---|---|---|---|
| MVPLAPO | x1/0.25 | 65 | 6.3x-63x |
| MVPLAPO | x2/0.5 | 20 | 12.5x-125x |
The x2 objective has a correction collar for correction of aberration produced by water or plastic container.
Light sources
Fluorescence: X-cite 120PC –Q-metal halide lamp
Incident Illumination: Dual Olympus tungsten-halogen dichroic reflector
Transmitted Illumination Base: LED four position turret (darkfield, brightfield, oblique).
Fluorescence Filters
| Filter | Excitation Filter | Dichroic | Emission Filter | Fluorophore Family |
|---|---|---|---|---|
| U-XL49000 | AT350/50 | T400LP | ET460/50 | DAPI |
| U-XL49002 | ET470/40 | T495LPXR | ET525/50 | Cy2,EGFP |
| U-XL49004 | ET545/25 | T565LPXR | ET605/70 | CY3,RFP |
Acquisition
Acquisition is performed with the Olympus proprietary Cell Sens Dimension software.
Output files can be opened with FIJI/ImageJ using the Olympus viewer plugin or exported to TIFF.
New users
Please contact Maya Holdengreber, tel. 073-3781107, to coordinate a meeting.
Reservations
The Olympus Stereoscope can be reserved in the BookItLab online reservation system.
