Widefield Zeiss Inverted

Introduction

Time Lapse 1 is an inverted system for transmitted and fluorescence widefield microscopy of fixed specimens and short or extended time lapse of live samples.

Hardware

The Zeiss Axio Observer microscope in Time lapse 1 system is wholly enclosed in a polycarbonate incubator system with controlled temperature and humidity and an active gas mixer. It features a motorized stage, X-cite metal halide light source and monochrome AxioCam HRm and high speed Axiocam HS.

Reflector Turret	Excitation	Beamsplitter	Emission	Suitable fluorophores
Analyzer module DIC
Zeiss Filter set 50 (optional)	BP 640/30	FT 660	BP 690/50	Cy5, DRAQ5
Zeiss Filter 20HE	BP 546/12	FT 560	BP 607/80	PI, Cy3, Alexa555, Rhodamine
Zeiss Filter set 38HE	BP 470/40	FT 495	BP 525/50	Cy2, GFP, Alexa 488
Zeiss Filter set 49	G 365	FT 395	BP 445/50	DAPI, Hoechst
Optovar x1.6

Objective	Magnification	NA	Contrast	Dish bottom or slide coverslip	Working distance (mm)	Immersion
EC Plan-Neofluar	x5	0.16	Phase 1	plastic/glass	18.5	air
EC Plan-Neofluar	x10	0.3	Phase 1	plastic/glass	5.2	air
Plan-Neofluar	x20	0.4	Phase 2	plastic/glass	7.4	air
Apochromat	x20	0.8	Phase 2 & DICII	Glass	0.55	air
Apochromat	x40	0.95	Phase 3	Glass	0.25	air
Plan-Apochromat (optional)	x63	1.4	Phase 3	Glass	0.19	oil

We have glass bottom dishes for you to try imaging with the higher-end objectives.

Acquisition

Acquisition is performed with the Zeiss proprietary Zen software. For basic visualization and processing, you can download the free ZEN lite edition software. Offline full versions are available for analysis at CT Computer and Computer IMARIS software. Output files can be opened by Imaris and ImageJ/FIJI for analysis.

Special applications

Long term live experiments in 6 dimensions: XYZ, positions, channels, time, in optimal gas composition or hypoxia. Also FRET, FRAP, wound healing, tube formation.

Analysis

For basic visualization and processing you can download the free ZEN lite edition software. Output files can be opened by ImageJ/FIJI and Imaris for analysis.

New users

Please contact Maya Holdengreber, tel. 073-378-1106, to coordinate a meeting.
Tips for immunofluorescence experiments.
Please prepare controls before starting a new model.

For reference you may use the Time Lapse 1 instruction file.

Reservations

For long term live widefield microscopy, the Time Lapse 1 Microscope can be reserved through the BookItLab online reservation system.