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Widefield Zeiss Upright
Introduction
An upright system for transmitted and fluorescence widefield microscopy of fixed specimens.
The Zeiss Axio Imager.Z2 upright microscope system is suited for fixed specimens. The system features a motorized stage and X-cite metal halide light illumination, a high-resolution monochrome Zeiss Axiocam 807, and a color Axiocam 105 camera.
Special applications
Tiling, positions, Z-stacks, color brightfield, polarized light.
Acquisition
The proprietary Zeiss Zen software provides fast acquisition, tiling and positions, multidimensional and fast acquisition and basic 2D analysis.
Hardware
Reflector Turret | Excitation | Beamsplitter | Emission | Suitable fluorophores |
Zeiss Filter set 49 | G 365 | FT 395 | BP 445/50 | DAPI, Hoechst |
Zeiss Filter set 45 | BP 560/40 | FT 585 | BP 630/75 | Mitotracker red, mCherry, Texas Red |
Zeiss Filter set 43 | BP 545/25 | FT 570 | BP 605/70 | Cy3, Mitotracker Orange, Rhodamin |
Chroma filter 49006 – ET – Cy5 | BP 620/60 | FT 660 | BP 700/75 | Cy5, DRAQ5 |
Zeiss Filter set 38 | BP 470/40 | FT 495 | BP 525/50 | Cy2, GFP, Alexa 488 |
Analyzer module DIC | ||||
Optovar x1.6 | ||||
Zeiss Filter set 47 (optional) | BP 436/20 | FT 455 | BP 480/40 | CFP |
Zeiss Filter Set 48 (optional) | BP 436/20 | FT 455 | BP 535/30 | CFP/YFP FRET |
Objective | Magnification | NA | Contrast | Coverslip | Working distance (mm) | Immersion |
EC Plan-Neofluar |
x5 |
0.16 | Phase 1 | plastic/glass |
18.5 |
air |
EC Plan-Neofluar |
x10 |
0.3 | Phase 1 | plastic/glass |
5.2 |
air |
Plan-Apochromat |
x20 |
0.8 | DICII | Glass |
0.55 |
air |
EC Plan-Neofluar | x40 | 0.75 | DICII | Glass | 0.71 | air |
Plan-Apochromat | x63 | 1.4 | DICIII | Glass | 0.19 | oil |
EC Plan Neofluar | x100 | 1.4 | BF | Glass | 0.17 | oil |
Analysis
For basic visualization and processing, you can download the free ZEN lite edition software. Offline full versions are available for analysis at CT Computer and Computer IMARIS software. Output files can be opened by Imaris and ImageJ/FIJI for analysis.
New users
Please contact Maya Holdengreber, tel. 073-378-1106, to coordinate a meeting.
Tips for immunofluorescence experiments.
Please prepare controls before starting a new model.
For reference you may use the Time Lapse 2 instruction file.
Reservations
For general widefield microscopy and for tiling, color brightfield, and polarized light applications, Microscope Time-lapse 2 can be reserved through the BookItLab online reservation system.