Widefield Zeiss Upright

Introduction

An upright system for transmitted and fluorescence widefield microscopy of fixed specimens.

The Zeiss Axio Imager.Z2 upright microscope system is suited for fixed specimens. The system features a motorized stage and X-cite metal halide light illumination, a high-resolution monochrome Hamamatsu Orca R2 camera, and a color Axiocam MRc 5 camera.

Special applications

Tiling, positions, Z-stacks, color brightfield, polarized light.

Acquisition

The proprietary Zeiss Zen software provides fast acquisition, tiling and positions, multidimensional and fast acquisition and basic 2D analysis.

Hardware

Reflector Turret	Excitation	Beamsplitter	Emission	Suitable fluorophores
Zeiss Filter set 49	G 365	FT 395	BP 445/50	DAPI, Hoechst
Zeiss Filter set 45	BP 560/40	FT 585	BP 630/75	Mitotracker red, mCherry, Texas Red
Zeiss Filter set 43	BP 545/25	FT 570	BP 605/70	Cy3, Mitotracker Orange, Rhodamin
Chroma filter 49006 – ET – Cy5	BP 620/60	FT 660	BP 700/75	Cy5, DRAQ5
Zeiss Filter set 38	BP 470/40	FT 495	BP 525/50	Cy2, GFP, Alexa 488
Analyzer module DIC
Optovar x1.6
Zeiss Filter set 47 (optional)	BP 436/20	FT 455	BP 480/40	CFP
Zeiss Filter Set 48 (optional)	BP 436/20	FT 455	BP 535/30	CFP/YFP FRET

Objective	Magnification	NA	Contrast	Coverslip	Working distance (mm)	Immersion
EC Plan-Neofluar	x5	0.16	Phase 1	plastic/glass	18.5	air
EC Plan-Neofluar	x10	0.3	Phase 1	plastic/glass	5.2	air
Plan-Apochromat	x20	0.8	DICII	Glass	0.55	air
EC Plan-Neofluar	x40	0.75	DICII	Glass	0.71	air
Plan-Apochromat	x63	1.4	DICIII	Glass	0.19	oil
EC Plan Neofluar	x100	1.4	BF	Glass	0.17	oil

Analysis

For basic visualization and processing, you can download the free ZEN lite edition software. Offline full versions are available for analysis at CT Computer and Computer IMARIS software. Output files can be opened by Imaris and ImageJ/FIJI for analysis.

New users

Please contact Maya Holdengreber, tel. 073-378-1106, to coordinate a meeting.
Tips for immunofluorescence experiments.
Please prepare controls before starting a new model.
For reference you may use the Time Lapse 2 instruction file.

Reservations

For general widefield microscopy and for tiling, color brightfield, and polarized light applications, Microscope Time-lapse 2 can be reserved through the BookItLab online reservation system.