Zeiss Celldiscoverer 7

Introduction

Celldiscoverer 7 is a fully integrated, automated high-content screening live cell inverted widefield imaging system for cell cultures, tissue sections or small model organisms with optional controlled environmental conditions including CO₂, O₂, N₂, heating and cooling. It features built-in automation for easy setup of complex experiments. High-resolution experiments can utilize automatic water immersion supply and removal which suits even long-term experiments.

Hardware

Cameras

The system has two monochrome cameras:

LED illumination and filter sets

LED combinations (nm)	Filter set	Beamsplitter	Emission filter
385 / 470 / 567 / 625 / IR-TL	90HE	405+493+575+653	425/30 + 514/30 + 592/25 + 709/100
420 / 520 / 590 / IR-TL	91HE	450+538+610	467/24 + 555/25 + 687/145
385 / 470 / 590 / IR-TL	92HE	405+493+610	425/30 + 524/50 + 688/145

Objective lenses with temperature control

Objective	Magnification	NA	Vessel thickness	Effective penetration	Working distance (mm)	Immersion
ZEISS Plan-APOCHROMAT 5× / 0.35	x5	0.35	Phase 1	up to 1.2mm		air
ZEISS Plan-APOCHROMAT 20× / 0.7	x20	0.7	Phase 1	0.13-1.2mm with autocorrection	1.33mm @ 0.17mm 0.4mm @ 1mm	air
ZEISS Plan-APOCHROMAT 20× / 0.95	x20	0.95	Phase 2	0.13-0.21mm with autocorrection	0.4mm @ 0.17mm	air
ZEISS Plan-APOCHROMAT 50× / 1.2 W	x50	1.2	Phase 2 & DICII	0.13-0.21mm with autocorrection	0.4mm @ 0.17mm	water autoimmersion

Sample mounting

One multiwell dish
One 35mm or 60mm petri dish
Three 76x26mm slides
Two slides/Lab-TekTM chambers 57×26 mm
Insert plate for perfusion with POC-R2

Acquisition and Analysis

Acquisition is performed with the Zen software which includes modules for automated quantification of fluorescence, cell confluence by transmitted light including wound healing and spot detection inside cell nuclei. For basic visualization and processing, you can download the free ZEN lite edition software. Offline full versions are available for analysis at CT Computer and Computer IMARIS software. Output files can be opened by Imaris and ImageJ/FIJI for analysis.

New users

Please contact Maya Holdengreber, tel. 073-378-1106, to coordinate a meeting.
Tips for immunofluorescence experiments.
Please prepare controls before starting a new model.

Reservations

For live widefield microscopy, the Celldiscoverer 7 can be reserved through the BookItLab online reservation system.