Incucyte SX5

Incucyte SX5

Introduction

The Sartorius Incucyte SX5 System is a high throughput, continuous live cell imager.

It enables kinetic transmitted (phase) and fluorescence imaging of long term live cell experiments inside the stable environment of an incubator without sacrificing cells to obtain data from each timepoint. The multiwell dish remains stationary and the microscope moves to image.

 

Hardware

Camera

Basler acA 1920-155um – Basler ace, with the Sony IMX174 CMOS sensor, 1920 px x 1200 px, 12bit.

Objectives

Objective Magnification/NA Pixel size
Nikon Plan Apo λ x4/0.20 2.82 μm
Nikon Plan Fluor x10/0.30 1.24 μm
Nikon S Plan Fluor x20/0.45 0.62 μm

All objectives have long working distance; many plastic off-the-shelf vessels can be used.

Channels

Channel Excitation Emission 
Green
Orange
NIR
Green
Orange
NIR
453-485 nm
546-568 nm
648-674 nm
494-533 nm
576-639 nm
685-756 nm
Green
Red
Green
Red
441-481 nm
567-607 nm
503-544 nm
622-704 nm
Metabolism ATP 473-498 nm
524-550 nm
565-591 nm
Orange
NIR
524-550 nm
648-674 nm
565-591 nm
685-756 nm

 

Acquisition

The proprietary software performs both acquisition and analysis. The software can be installed on any computer in the Technion and the experiment can be monitored in real time. The acquired images can be exported to JPEG, TIFF, TIFF series, MPEG4, AVI and the graphs can be exported as excel files or graphs.

 

Applications

Cell Counting and Proliferation
Viability
Apoptosis
Cytotoxicity
Tumor Spheroid
Cell Cycle
ATP Metabolism
Mitochondrial Membrane Potential
Immune Cell Killing
Antibody Internalization
Phagocytosis
NETosis
Neuronal Activity
Angiogenesis
Live-Cell Immunocytochemistry
Scratch Wound Migration
Invasion
Chemotaxis
Immune Cell Activation

 

New users

Please contact Maya Holdengreber, tel. 073-3781106, to coordinate a meeting.

 

Reservations

The Incucyte SX5 can be reserved through our BookItLab online reservation system.