The Olympus IXplore Spin microscope system’s spinning disk is a system which combines optical sectioning of confocal with the high sensitivity of widefield microscopy allowing imaging of fast dynamic processes and live specimens while considerably reducing phototoxicity. The CSUW1-T1S spinning disk supports an especially large field of view (17mm x 16mm), fast 3D confocal image acquisition, and prolonged cell viability in time-lapse experiments.
OBIS LX laser system 405nm, 50mW
OBIS LX laser system 488nm, 100mW
OBIS LX laser system 561nm, 100mW
OBIS LX laser system 640nm, 100mW
B447/60, B525/50, B617/73, B685/40
Images are acquired with a Prime BSI Express monochrome camera.
True color white LED light source 400-635nm excitation and triple-band filter for DAPI/FITC/TRITC.
Universal Plan Extended Apochromat Objective lenses
| Objective | Magnification | NA | Coverslip Thickness | Working distance (mm) | Immersion |
|---|---|---|---|---|---|
| UPLXAPO 4x | x4 | 0.16 | any | 13mm | air |
| UPLXAPO 10x | x10 | 0.4 | 0.17mm | 3.1mm | air |
| UPLXAPO 20x | x20 | 0.8 | 0.17mm | 0.6mm | air |
| UPLXAPO 60x | x60 | 1.42 | 0.17mm | 0.15mm | oil |
LCI Incubator System T including CO2/Air gas mixer, temperature controller, lens warmer, humidifier for 35mm petri dishes or 75 x 25mm multichamber or slide.
Acquisition is performed with the CS-DI-v4.1 cellSens Dimension v4 software.
Please contact Maya Holdengreber, tel. 073-378-1106, to coordinate a meeting.
Tips for immunofluorescence experiments.
Please prepare controls before starting a new model.
For spinning disk confocal microscopy, the Olympus IXplore Spin can be reserved through the BookItLab online reservation system.
