The FMC center houses a FACSAria™ IIIu (from BD Biocsiences) instrument, a digital high-speed cell sorter. It is equipped with 5 lasers lines; 375nm, 405nm, 488nm, 561nm and 633nm) with 11 fluorescence detectors for use in complex cell sorting plus forward and side scatter. We support 2- and 4-way sorting into 1.5ml, 5ml or 15 ml collecting tubes, with four sizes of nozzles (70, 85, 100 and 130 micron) to accommodate the wide range of particle sizes. In addition, the Automated Cell Deposition Unit (ACDU) option is available for sorting directly into multi-well plates or onto microscope slides. We also have a temperature controlled sample chamber and collection tube holder to support cell viability and an Aerosol Management Option (AMO) for biohazard containment.
For filters and channel information see here.
Pre-reservation is required via BookIt. Please make sure the availability of our staff prior reserving your session.
The Aria is technician operated and is available from 8am to 18pm Sunday to Thursday. During these hours, the Aria will be setup for you by the FMC staff based on your needs documented on the BookIt reservation. Operation outside normal times may be possible by prior arrangement – For further information or if you need assistance please contact core personnel.
please see our Beginner’s Guide on “How to bring your cells for sorting” for some tips and tricks.