IVIS Lumina X5

Introduction

The PerkinElmer IVIS Lumina X5 combines high-throughput in vivo bioluminescence and fluorescence imaging with high-resolution 2D X-ray.

The IVIS Lumina X5 instrument provides 20 x 20 cm field of view, for imaging up to 5 animals at a time, ex-vivo specimens and cell culture plates with bioluminescence and fluorescence. A large, independently deployed scintillator enables X-ray acquisitions of large rodents, up to 600 grams or 5 mice.

The IVIS Lumina X5 is equipped with 26 filters (19 excitation filters and 7 emission filters) to image fluorescent sources that emit from green to near-infrared. The X-ray module employs automated image integration to overlay with bioluminescence, fluorescence and photography.

The system is equipped with an isoflurane anesthesia system, low-autofluorescence optics and a cooled CCD camera (‑90°C) for a maximum resolution of 50µm.

 

Available fluorescence filters

Excitation filters

Emission filters

Center (nm)

Passband (nm) Center (nm)

Passband (nm)

420

20 520

40

440

20 570

40

460

20 620

40

480

20 670 40

500

20 710

40

520

20 790

40

540

20 845

40

560

20  

 

580

20  

 

600

20  

 

620

20  

 

640

20  

 

660

20  

 

680

20  

 

700

20  

 

720

20  

 

740

20  

 

760

20  

 

780

20  

 

 

Applications

  • Quantitative longitudinal measurement of tumor development, metastasis, angiogenesis, and response to therapy
  • Trafficking of cellular and other biological therapies (e.g., immune cell, stem cell, or viral therapeutic platforms or antibodies, peptides, metabolites, etc.)
  • Monitoring of host immune cell responses
  • Distribution, quantification, and kinetic analyses of gene expression or enzymatic activity in vivo

Analysis

The IVIS Lumina X5 Living Image software (Living Image 4.5) allows absolute quantification (photons/sec/cmsq/sr) of bioluminescence and fluorescence signal, for in vivo, ex vivo and in vitro studies.

The software supports accurate autofluorescence removal, unmixing, fluorophore quantitation, automatic ROI creation, background subtraction and scaling.

 The software is available both at the Lumina X5 station and at the BCF image analysis unit on our Computer in-vivo analysis.

New users

Please contact Edith Suss-Toby, tel. +972-4-8295347/61 to coordinate a meeting.

Reading materials prior to the first instruction session:

IVIS 200 Basics

Living Image 4.5.4 User manual

The system can be reserved in the BookItLab online ordering system, In-vivo imaging IVIS Lumina X5; luciferin aliquots (15mg/ml) can be ordered in BookItLab, under Other Services; select Offline service type: Luciferin tube

Protocol for in vitro bioluminescence assay