Widefield Zeiss Upright

Introduction

An inverted system for transmitted and fluorescence widefield microscopy of fixed specimens.

The Zeiss Axio Imager.Z2 upright microscope system is suited for fixed specimens. The system features a motorized stage and X-cite metal halide light illumination, high resolution monochrome Hamamatsu Orca R2 camera , and color Axiocam MRc 5 camera. 

Reflector Turret Excitation Beamsplitter Emission Suitable fluorophores
Zeiss Filter set 49 G 365 FT 395 BP 445/50 DAPI, Hoechst
Zeiss Filter set 45 BP 560/40 FT 585 BP 630/75 Mitotracker red, mCherry, Texas Red
Zeiss Filter set 43 BP 545/25 FT 570 BP 605/70 Cy3, Mitotracker Orange, Rhodamin
Chroma filter 49006 – ET – Cy5 BP 620/60 FT 660 BP 700/75 Cy5, DRAQ5
Zeiss Filter set 38 BP 470/40 FT 495 BP 525/50 Cy2, GFP, Alexa 488
Analyzer module DIC
Optovar x1.6
Zeiss Filter set 47 (optional) BP 436/20 FT 455 BP 480/40 CFP
Zeiss Filter Set 48 (optional) BP 436/20 FT 455 BP 535/30 CFP/YFP FRET

 

Objective Magnification NA Contrast Dish bottom or slide coverslip Working distance (mm) Immersion
EC Plan-Neofluar x5 0.16 Phase 1 plastic/glass 18.5 air
EC Plan-Neofluar x10 0.3 Phase 1 plastic/glass 0.3 air
Plan-Apochromat x20 0.8 DICII Glass 0.55 air
EC Plan-Neofluar x40 0.75 DICII Glass 0.71 air
Plan-Apochromat x63 1.4 DICIII Glass 0.19 oil
EC Plan Neofluar x100 1.4 BF Glass 0.17 oil

 

Special applications

Tiling, positions, Z-stacks, color brightfield.

Acquisition

The proprietary Zeiss Zen software provides fast acquisition, tiling and positions, multidimensional and fast acquisition and basic 2D analysis.

Analysis 

For basic visualization and processing you can download the free ZEN lite edition software. Output files can be opened by ImageJ/FIJI and Imaris for analysis.

 

New users

Please contact Maya Holdengreber, tel. +972-77-8871107 to coordinate a meeting.

Tips for immunofluorescence experiments.

Please prepare controls before starting a new model.

For reference you may use the Time Lapse 2 instruction file.

Reservations

For general widefield microscopy and for tiling, color brightfield, Time Lapse 2 can be reserved in the BookItLab online reservation system, Microscope Time-lapse 2.

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