Introduction
An inverted system for transmitted and fluorescence widefield microscopy of fixed specimens.
The Zeiss Axio Imager.Z2 upright microscope system is suited for fixed specimens. The system features a motorized stage and X-cite metal halide light illumination, high resolution monochrome Hamamatsu Orca R2 camera , and color Axiocam MRc 5 camera.
| Reflector Turret | Excitation | Beamsplitter | Emission | Suitable fluorophores |
|---|---|---|---|---|
| Zeiss Filter set 49 | G 365 | FT 395 | BP 445/50 | DAPI, Hoechst |
| Zeiss Filter set 45 | BP 560/40 | FT 585 | BP 630/75 | Mitotracker red, mCherry, Texas Red |
| Chroma 49005 | ET 545/30 | T 570 LP | ET 620/60 | Cy3, Mitotracker Orange, DsRed |
| Zeiss Filter 20HE | BP 546/12 | FT 560 | BP 607/80 | PI, Cy3, Alexa555, Rhodamine |
| Zeiss Filter set 38HE | BP 470/40 | FT 495 | BP 525/50 | Cy2, GFP, Alexa 488 |
| Analyzer module DIC | ||||
| Optovar x1.6 | ||||
| Zeiss Filter set 47 (optional) | BP 436/20 | FT 455 | BP 480/40 | CFP |
| Zeiss Filter Set 48 (optional) | BP 436/20 | FT 455 | BP 535/30 | CFP/YFP FRET |
| Objective | Magnification | NA | Contrast | Dish bottom or slide coverslip | Working distance (mm) | Immersion |
|---|---|---|---|---|---|---|
| EC Plan-Neofluar | x5 | 0.16 | Phase 1 | plastic/glass | 18.5 | air |
| EC Plan-Neofluar | x10 | 0.3 | Phase 1 | plastic/glass | 0.3 | air |
| Plan-Apochromat | x20 | 0.8 | DICII | Glass | 0.55 | air |
| EC Plan-Neofluar | x40 | 0.75 | DICII | Glass | 0.71 | air |
| Plan-Apochromat | x63 | 1.4 | DICIII | Glass | 0.19 | oil |
| EC Plan Neofluar | x100 | 1.4 | BF | Glass | 0.17 | oil |
Special applications
Tiling, positions, Z-stacks, color brightfield.
Acquisition
The proprietary Zeiss Zen software provides fast acquisition, tiling and positions, multidimensional and fast acquisition and basic 2D analysis.
Analysis
For basic visualization and processing you can download the free ZEN lite edition software. Output files can be opened by ImageJ/FIJI and Imaris for analysis.
Instructions for specific applications
New users
Please contact Edith Suss-Toby, tel. +972-4-8295347/61 to coordinate a meeting.
Tips for immunofluorescence experiments.
Please prepare controls before starting a new model.
For reference you may use the Time Lapse 2 instruction file.
Reservations
For general widefield microscopy and for tiling, color brightfield, Time Lapse 2 can be reserved in the BookItLab online reservation system, Microscope Time-lapse 2.
