Introduction
The Olympus IXplore Spin microscope system’s spinning disk is a system which combines optical sectioning of confocal with the high sensitivity of widefield microscopy allowing imaging of fast dynamic processes and live specimens while considerably reducing phototoxicity. The CSUW1-T1S spinning disk supports an especially large field of view (17mm x 16mm), fast 3D confocal image acquisition, and prolonged cell viability in time-lapse experiments.
Lasers
OBIS LX laser system 405nm, 50mW
OBIS LX laser system 488nm 100mW
OBIS LX laser system 561nm, 100mW
OBIS LX laser system 640nm, 100mW
Cameras
Images are acquired with a Prime BSI Express monochrome camera.
LED illumination
True color white LED light source 400-635nm excitation and triple-band filter for DAPI/FITC/TRITC.
Universal Plan Extended Apochromat Objective lenses
| Objective | Magnification | NA | Coverslip Thickness | Working distance (mm) | Immersion | |
|---|---|---|---|---|---|---|
| UPLXAPO 4x | x4 | 0.16 | any | 13mm | air | |
| UPLXAPO 10x | x10 | 0.4 | 0.17mm | 3.1mm | air | |
| UPLXAPO 20x | x20 | 0.8 | 0.17mm | 0.6mm | air | |
| UPLXAPO 60x | x60 | 1.42 | 0.17mm | 0.15mm | oil |
Sample mounting
Ultimage Incubator System including CO2/Air gas mixer, temperature controller, lens warmer, humidifier for 35mm petri dishes or 75 x 25mm multichamber or slide.
Acquisition and Analysis
Acquisition is performed with the CS-DI-v4.1 cellSens Dimension v4 software.
New users
Please contact Maya Holdengreber, tel. +972-73-3781106, to coordinate a meeting.
Tips for immunofluorescence experiments.
Please prepare controls before starting a new model.
Reservations
For spinning disk confocal microscopy, the Olympus IXplore Spin can be reserved in the BookItLab online reservation system following an instruction session.