Widefield inverted

Introduction

Time Lapse 1 is an inverted system for transmitted and fluorescence widefield microscopy of fixed specimens and short or extended time lapse of live samples.

Hardware specifications

The Zeiss Axio observer microscope in Time lapse 1 system is wholly enclosed in a polycarbonate incubator system with controlled temperature and humidity and an active gas mixer. It features a motorized stage, X-cite metal halide light source and monochrome AxioCam HRm and high speed Axiocam HS. 

 

Reflector Turret Excitation Beamsplitter Emission Suitable fluorophores
Analyzer module DIC
Zeiss Filter set 50 (optional) BP 640/30 FT 660 BP 690/50 Cy5, DRAQ5
Zeiss Filter 20HE BP 546/12 FT 560 BP 607/80 PI, Cy3, Alexa555, Rhodamine
Zeiss Filter set 38HE BP 470/40 FT 495 BP 525/50 Cy2, GFP, Alexa 488
Zeiss Filter set 49 G 365 FT 395 BP 445/50 DAPI, Hoechst
Optovar x1.6

 

Objective Magnification NA Contrast Dish bottom or slide coverslip Working distance (mm) Immersion
EC Plan-Neofluar x5 0.16 Phase 1 plastic/glass 18.5 air
EC Plan-Neofluar x10 0.3 Phase 1 plastic/glass 0.3 air
Plan-Neofluar x20 0.4 Phase 2 plastic/glass 0.4 air
Apochromat x20 0.8 Phase 2 & DICII Glass 0.55 air
Apochromat x40 0.95 Phase 3 Glass 0.25 air
Plan-Apochromat (optional) x63 1.4 Phase 3 Glass 0.19 oil

 

We have glass bottom dishes for you to try imaging with the higher end objectives 

Acquisition

Acquisition is performed with the Zeiss proprietary Zen software. Output files can be opened by the ZEN lite edition softwareImageJ/FIJI or exported to TIFF for analysis.

The Zen 2 software provides fast acquisition, tiling and positions, multidimensional and fast acquisition and basic 2D analysis.

Special applications

Long term live experiments in 6 dimensions: XYZ, positions, channels, time, in optimal gas composition or hypoxia. Also FRET, FRAP, wound healing, tube formation. 

Analysis 

For basic visualization and processing you can download the free ZEN lite edition software. Output files can be opened by ImageJ/FIJI and Imaris for analysis.

 

Instructions for specific applications

New users

Please contact Edith Suss-Toby, tel. +972-4-8295347/61 to coordinate a meeting.

Tips for immunofluorescence experiments.

Please prepare controls before starting a new model.

For reference you may use the Time Lapse 1 instruction file .

Reservations

For long term live widefield microscopy, Time Lapse 1 can be reserved in the BookItLab online reservation system, Microscope Time-lapse 1.

 

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